乳腺癌耐药蛋白ABCG2对胰腺癌SW1990细胞耐药性影响的实验

王飞通; 周兵; 刘小云; 汪正伟; 牛坚; 魏鑫; 刘斌

doi:10.3971/j.issn.1000-8578.2014.10.003

乳腺癌耐药蛋白ABCG2对胰腺癌SW1990细胞耐药性影响的实验

Effect of ABCG2 Expression on Chemoresistance of Pancreatic Carcinoma Cell Line SW1990

摘要

摘要: 目的探讨吉西他滨诱导胰腺癌细胞SW1990中ABCG2的表达及其与化疗耐药的关系。方法胰腺癌细胞SW1990用DMEM培养液常规培养，用0.82 mg/ml吉西他滨作用SW1990细胞24、48和72 h后，使用流式细胞仪测其细胞凋亡率，Western blot检测ABCG2蛋白的表达，RT-PCR检测ABCG2 mRNA的表达，并且分析ABCG2表达水平与化疗耐药的关系。结果 0.82 mg/ml吉西他滨作用SW1990细胞24、48和72 h后，细胞凋亡率分别为（21.1±0.61）%、（13.4±2.17）%、（6.4±1.34）%，不同时间点两两相比P均<0.05。0.82 mg/ml吉西他滨作用SW1990细胞24、48、72 h后与对照组相比，ABCG2 mRNA分别上升了（2.21±0.11）倍、（3.30±0.08）倍和（4.72±0.12）倍，蛋白上升了（2.17±0.14）倍、（3.61±0.09）倍和（4.98±0.13）倍，且组间比较P均<0.05，有统计学意义。结论吉西他滨能抑制胰腺癌细胞SW1990的增殖，但随着时间延长，药物抵抗逐渐增强，这可能与吉西他滨作用细胞后上调ABCG2的表达有关。

Abstract: Objective To explore the relationship between chemoresistance to gemcitabine and ABCG2 expression in pancreatic carcinoma cell line SW1990. Methods The pancreatic carcinoma cell line SW1990 was cultured by DMEM and treated with 0.82 mg/ml gemcitabine for 24, 48 and 72 h. Then apoptosis rate was measured by flow cytometry, ABCG2 expression was examined by Western blot and ABCG2 mRNA expression was detected by RT-PCR. The relationship between ABCG2 expression and chemoresistance to gemeitabine was analyzed. Results After treated with 0.82 mg/ml gemeitabine for 24, 48 and 72 h, the apoptosis rates were(21.1±0.61)%, (13.4±2.17)%, (6.4±1.34)%, respectively (comparison between different time points, P<0.05); moreover, compared with control group, ABCG2 mRNA expression were increased by(2.21±0.11), (3.30±0.08) and (4.72±0.12) times and the protein expression were increased by(2.17±0.14), (3.61±0.09) and (4.98±0.13) times respectively (comparison between groups, P<0.05). Conclusion Gemcitabine could inhibit cell proliferation of pancreatic carcinoma cell line SW1990, but the chemoresistance would be enhanced gradually with time, which may be related with increased ABCG2 expression.

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