Transforming Growth Factor-β Induced Gene Inhibits Cell Proliferation of Malignant Mesothelioma in vitro
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摘要: 目的 研究转化生长因子β诱导的基因 (transforming growth factor-β induced gene, TGFBI) 在体外是否能抑制恶性间皮瘤细胞NCI-H28的增殖。方法 将外源性TGFBI稳定性转染到恶性间皮瘤细胞NCI-H28中，绘制生长曲线图，测定其细胞增殖、接种效率和软琼脂克隆形成，并进行细胞周期的分析。结果 外源性TGFBI在恶性间皮瘤细胞NCI-H28中能够稳定地高表达；当外源性的TGFBI 转入到肿瘤细胞NCI-H28后，TGFBI高表达的细胞倍增时间增加了4.38倍；与转染空载体的肿瘤细胞相比：NCI-H28的相对接种效率降低了69.68 %，外源性TGFBI表达的T28细胞形成了较小的软琼脂克隆；TGFBI可以使肿瘤细胞阻滞在G1期，并延缓肿瘤细胞进入S期的时间。结论 TGFBI可以抑制恶性间皮瘤细胞的体外增殖。Abstract: Objective To explore that transforming growth factor-β induced gene (TGFBI) inhibits NCI-H28 growth in vitro. Methods TGFBI was stably transfected into mesothelioma cell line NCI-H28. growth curve, cell proliferation, platting effi ciency, and colony formation in soft agar were analyzed. Results Exogenous TGFBI in malignant mesothelioma NCI-H28 had a high expression in stably. After exogenous TGFBI transferred into tumor cells NCI-H28, doubling time of TGFBI high expression cell was increased by 4.38 times, compared with the empty vector. Relative plating effi ciency of NCI-H28 was decreased by 69.68%. T28 cells with exogenous TGFBI formed a smaller soft agar; TGFBI made tumor cells arrest in G1 phase and delay tumor cells into the S phase . Conclusion TGFBI was able to inhibit the cell proliferation of malignant mesothelioma in vitro.
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