抑制PI3K/Akt通路提高HeLa细胞化疗效果的实验研究

Improving Chemotherapy Effect of HeLa Cells by Inhibiting PI3K/Akt Signal Transduction

摘要: 目的探讨抑制PI3K/Akt信号转导通路提高抗癌药物对人宫颈癌HeLa细胞的杀伤作用。方法采用MTT法检测Celecoxib、DDP及Docetaxel单独或联合PI3K抑制剂LY294002对人宫颈癌HeLa细胞的抑制率;采用流式细胞技术检测药物单独或联合作用对HeLa细胞凋亡的影响。结果(1)联合LY294002能够显著提高Celecoxib、DDP及Docetaxel对HeLa细胞抑制率;(2)LY294002与Celecoxib、DDP及Docetaxel的协同治疗指数均小于1,二者起协同治疗作用;(3)联合LY294002能够增加HeLa细胞的凋亡水平。结论抑制PI3K/Akt信号转导通路能够显著提高Celecoxib、DDP及Docetaxel对HeLa细胞杀伤作用。

Abstract: Objective To explore the lethal effect role of the therapeutic alliance of inhibiting PI3K/Akt signal transduction and anticancer drugs in HeLa cells of cervix cancer. Methods Detect the inhibition ratio of celecoxib, DDP and docetaxel in Hela or associated with PI3K inhibitor-LY294002 by Mono-nuclear cell direct cytotoxicity assay (MTT). Detect the apoptosis ratio of Celecoxib, DDP and Docetaxel in Hela or associated with PI3K inhibitor-LY294002 by flow cytometer. Results 1. The inhibition ratio was enhanc...