Abstract: Objective To explore the significance of DNA quantitative cytology and High-risk HPV-DNA testing in ASCUS riage diagnosis. Methods The DNA quantitative cytology group included 193 cases and the high-risk HPV-DNA comprises 134 cases.ASCUS diagnosed by TBS undergone with automatic cell tumour check analysis system or High-risk HPV-DNA test later, were both done colposcopy directed biopsy soon.There was a comparison between these two cases in pathological results. Results Of the 327 ASCUS cases, 204 cases were histologically diagnosed as inflammation （62.39%）, 70 cases were CIN Ⅰ(21.41%), 13 cases were CINⅡ（3.98%)， 30 cases were CIN Ⅲ (9.17％),and 10 cases were cervical invasive cancer (3.05%).The histological diagnosis were no statistical distinction between HR-HPV-DNA and DNA Quantitative cytology groups. The negative predictive values of HPV-DNA and DNA Quantitative cytology were 94.74% and 98.93％,respectively. Compared with the percentage of DNA amount greater than 5C (DNA Index>2.5) cells, the histological diagnosis were significantly different between ＜0.1％ and ≥0.1% .DNA heteroploid more than or equal to three were used to predict CINⅡor more severe pathological changes, and its sensitivity, specificity, positive predictive values and negative predictive values were 80.64％,47.06％,40.99％,84.21％, respectively. Conclusion DNA Quantitative cytology and High-risk HPV-DNA test can be used for ASCUS triage, but the cost of DNA Quantitative cytology is cheaper than High-risk HPV-DNA test.