Abstract: Objective To investigate the effect of LPS stimulation on the active release of HMGB1 in Cervical cancer cells and the ability of invasion and migration. Methods Three cell lines were cultured in DMEM: HeLa cell lines，C33A cell lines and HUCEC cell lines （Normal cervical epithelial cells）.Western blot was used to detect the intracellular and extracellular protein expression changes of HMGB1 before and after LPS added to the three cell lines.Transwell assay was used to compare the ability of invasion and migration before and after LPS added. Results After stimulated with 100 ng/ml LPS,intracellular total protein HMGB1 all decreased in the three cells,while the expression of HMGB1 in the supernatant increased（P<0.05）;the ability of invasion and migration in HeLa and C33A cancer cells significantly enhanced （P<0.05）. Conclusion LPS could facilitate the active release of HMGB1 in cervical cancer cells, and therefore enhanced the ability of invasion and migration.