Expression of Beclin1 in Fine Needle Aspiration Breast Lesions and Its Relationship with Bcl-2 and p53
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摘要: 目的 检测细胞自噬基因Beclin1在细针穿刺乳腺良恶性病变中的表达及其与Bcl-2、p53的关系，探讨其在乳腺癌发生发展中的作用及其机制。方法 采用RT-PCR和免疫组织化学检测乳腺良恶性病变中Beclin1、Bcl-2、p53mRNA及蛋白表达水平。结果 Beclin1 mRNA在乳腺癌中的表达明显低于在乳腺良性病变中的表达（P<0.05），Bcl-2、p53 mRNA在乳腺癌中的表达高于在乳腺良性病变中的表达（P<0.05）。Beclin1蛋白在乳腺癌中的阳性表达率明显低于在乳腺良性病变中的阳性表达率（P<0.05）；Bcl-2、p53蛋白在乳腺癌中的阳性表达率明显高于在乳腺良性病变中的阳性表达率（P<0.05）；在乳腺癌中Beclin1蛋白表达与Bcl-2和p53蛋白表达之间存在负相关（P<0.05）；而Bcl-2和p53蛋白的表达无相关性（P>0.05）。结论 Beclin1在乳腺癌组织中表达下调，而Bcl-2、p53在乳腺癌中均有高表达。Beclin1蛋白与Bcl-2、p53蛋白在乳腺癌组织中的表达存在负相关。Abstract: Objective To Explore the expression of Beclin1 in fine needle aspiration breast lesions, and its relationship with Bcl-2 and p53, to discuss the role of autophagy related gene-Beclin1 in breast cancer development. Methods RT-PCR and immunohistochemistry were used to detect the mRNA and protein expression of Beclin1, Bcl-2，p53 in breast lesions. Results The expression of Beclin1 mRNA in breast cancers was lower than those in benign breast lesions (P<0.05);the expressions of Bcl-2 and p53 mRNA in breast cancers were higher than those in benign breast lesions (P<0.05).The positive expression rate of Beclin1 in breast cancers was lower than that in benign breast lesions（P<0.05);the positive expression rates of Bcl-2 and p53 in breast cancers were higher than those in benign breast lesions（P<0.05）; there were negative correlations between Beclin1 and p53,Bcl-2 (P<0.05); no correlation was found between p53 and Bcl-2 (P>0.05). Conclusion The low expression of Beclin1 and the high expression of p53,Bcl-2 is indentified in breast cancers.There are negative correlations between Beclin1 and p53, Bcl-2 in breast cancers.
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