Abstract: Objective To Explore the expression of Beclin1 in fine needle aspiration breast lesions, and its relationship with Bcl-2 and p53, to discuss the role of autophagy related gene-Beclin1 in breast cancer development. Methods RT-PCR and immunohistochemistry were used to detect the mRNA and protein expression of Beclin1, Bcl-2，p53 in breast lesions. Results The expression of Beclin1 mRNA in breast cancers was lower than those in benign breast lesions (P<0.05);the expressions of Bcl-2 and p53 mRNA in breast cancers were higher than those in benign breast lesions (P<0.05).The positive expression rate of Beclin1 in breast cancers was lower than that in benign breast lesions（P<0.05);the positive expression rates of Bcl-2 and p53 in breast cancers were higher than those in benign breast lesions（P<0.05）; there were negative correlations between Beclin1 and p53,Bcl-2 (P<0.05); no correlation was found between p53 and Bcl-2 (P>0.05). Conclusion The low expression of Beclin1 and the high expression of p53,Bcl-2 is indentified in breast cancers.There are negative correlations between Beclin1 and p53, Bcl-2 in breast cancers.