高级搜索

EB病毒抗体检测在不同年龄壮族鼻咽癌患者中的应用

李晓华, 蒙以良, 赵丽娟, 黄承乐, 黄钜略, 漆光紫

李晓华, 蒙以良, 赵丽娟, 黄承乐, 黄钜略, 漆光紫. EB病毒抗体检测在不同年龄壮族鼻咽癌患者中的应用[J]. 肿瘤防治研究, 2013, 40(04): 377-380. DOI: 10.3971/j.issn.1000-8578.2013.04.014
引用本文: 李晓华, 蒙以良, 赵丽娟, 黄承乐, 黄钜略, 漆光紫. EB病毒抗体检测在不同年龄壮族鼻咽癌患者中的应用[J]. 肿瘤防治研究, 2013, 40(04): 377-380. DOI: 10.3971/j.issn.1000-8578.2013.04.014
shu
LI Xiaohua, MENG Yiliang, ZHAO Lijuan, HUANG Chengle, HUANG Julue, QI Guangzi. Epstein-barr Virus Antibodies Response for Zhuang NPC Patients of Different Ages[J]. Cancer Research on Prevention and Treatment, 2013, 40(04): 377-380. DOI: 10.3971/j.issn.1000-8578.2013.04.014
Citation: LI Xiaohua, MENG Yiliang, ZHAO Lijuan, HUANG Chengle, HUANG Julue, QI Guangzi. Epstein-barr Virus Antibodies Response for Zhuang NPC Patients of Different Ages[J]. Cancer Research on Prevention and Treatment, 2013, 40(04): 377-380. DOI: 10.3971/j.issn.1000-8578.2013.04.014
shu

EB病毒抗体检测在不同年龄壮族鼻咽癌患者中的应用

  • 1.533000 广西百色，广西右江民族医学院微生物学与免疫学教研室；2.广西百色市人民医院肿瘤科，3.检验科；4.广西右江民族医学院预防医学教研室

详细信息
    作者简介:

    李晓华（1979-），女，硕士，讲师，主要从事微生物学与肿瘤免疫学研究

    通讯作者:

    赵丽娟，E-mail：zhaolijuanxk@163.com

  • 中图分类号: R739.63

Epstein-barr Virus Antibodies Response for Zhuang NPC Patients of Different Ages

  • 1.Department of Microbiology and Immunology，Youjiang Medical College for Nationalities, Baise 533000, China；2. Department of Oncology, The People's Hospital of Baise City,3.Department of Laboratory；4.Department of Preventive Medicine，Youjiang Medical College for Nationalities

摘要

    摘要
  • 摘要: 目的 探讨桂西地区壮族鼻咽癌患者EB病毒各年龄段Rta/IgG、VCA/IgA、VCA/IgG及Zta/IgG抗体的rA值和阳性率与年龄的关系。 方法 收集140 例未经治疗的鼻咽癌患者和280例健康人的血清，用酶联免疫吸附法（ELISA）检测Rta/IgG、VCA/IgA、VCA/IgG及Zta/IgG抗体，分别计算各年龄段的抗体水平及阳性率并进行统计学分析。 结果 鼻咽癌患者各年龄段VCA/IgA抗体rA值和阳性率差异均有统计学意义 (P<0.05)。在50岁以后年龄段的患者与健康人Rta/IgG、VCA/IgA抗体阳性率比较差异有统计学意义 (P<0.05)。在50岁以后的年龄段患者与健康人Zta/IgG抗体阳性率比较差异无统计学意义 (P>0.05)。 结论 壮族鼻咽癌患者与健康人EB病毒Rta/IgG、VCA/IgA及Zta/IgG抗体水平和阳性率比较存在年龄上的差异。在鼻咽癌的临床诊断和高危人群筛查中有必要根据不同年龄段的人群界定不同的阳性临界值。

     

    Abstract: Objective To evaluate the relationship between ages and the Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG values and positive rate of the Epstein-Barr virus (EBV) antibodies in Zhuang NPC patients at different ages in west Guangxi. Methods Serum samples obtained from140 patients with nasopharyngeal carcinoma (NPC) and from 280 healthy people were examined for the EBV antibodies including Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG by the enzyme-linked immnunosorbent assay (ELISA).The positive rates and antibody levels of the NPC patients at different ages were analyzed statistically. Results Significant difference in VCA/IgA values and positive rates was found in the NPC patients at different ages (P<0.05).There was significant difference in the positive rates of Rta/IgG and VCA/IgA between the patients and healthy people above 50 years old (P<0.05).But no significant difference in the positive rates of Zta/IgG was found between them (P>0.05). Conclusion The serum levels and positive rates of EB virus antibodies (Rta/IgG,VCA/IgA and Zta/IgG) fluctuate with different ages. Different positive critical values of EBV antibodies for respective age groups should be set to facilitate NPC diagnosis and high-risk screening.

     

    • HTML全文
    • 参考文献(10)
  • [1] Ren J,Zhang XM,Zhang XG,et al.Study on antibody response to recombinant Rta protein in patient with nasopharyngeal carcinoma[J].Zhonghua Wei Sheng Wu Xue He Mian Yi Xue Za Zhi,2006,26(11):1057-9.[任军,张晓梅,张晓光,等.以Rtac2/3为抗原用于鼻咽癌病人检测的初步研究[J].中华微生物学和免疫学杂志,2006,26(11):1057-9. ]
    [2] Cheng MT,Cheng WM,Ji MF,et al.Detection of serum Epstein-Barr virus antibody level by ELISA in normal populations and nasopharyngeal carcinoma patients in Zhongshan City of China[J].Di Yi Jun Yi Da Xue Xue Bao,2002,22(9):817-22.[程敏婷,程伟民,季明芳,等.ELISA法检测中国中山市正常人及鼻咽癌患者血清EB病毒抗体水平[J].第一军医大学学报,2002,22(9):817-22.]
    [3] Cheng WM, Chan KH, Chen HL, et al. Assessing the risk of nasopharyngeal carcinoma on the basis of EBV antibody spectrum[J]. Int J Cancer, 2002, 97(4): 489-92.
    [4] Wu WH,Chen GX,Wu ZB,et al.Epsttein-Barr virs serology in early detection and screening of nasopharyngeal crcinoma[J].Ai Zheng,2006,25(2):250-6.[吴文翰,陈国雄,吴子柏,等.早期发现和筛选鼻咽癌的EB病毒血清学检测[J].癌症,2006,25(2):250-6.]
    [5] Feederle R, KostM, Baumann M, et al.The Epstein-Barr virus lytic program is controlled by the co-operat ive functions of two transact ivators[J].EMBO J,2000,19(12):3080-9.
    [6] Liu P, Speck SH.Synergistic autoactivation of the Epstein-Barr virus the immediate-early BRLF1 promoter by Rta and Zta[J].Virology, 2003,310(2):199-206.
    [7] Rooney CM, Rowedt , Ragot T , et al. The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle [J].J Virol, 1989,63(7): 3109-16.
    [8] Dardari R,Hinderer W,Lang D,et al.Antibody responses to recombinant Epstein-Barrvirus antigens in nasopharyngeal carcinoma patients:complementary test of ZEBRA protein and early antigens p54 and p138[J].J Clin Microbiol,2001,39(9):3164-70.
    [9] Liu Y,Hu CH,Yin RX,et al.Infection situation analysis of serum Epsttein-Barr virs in minorities and Han nationality from Luocheng county[J].Chongqing Yi Xue,2012,41(14):1404-6.[刘岩,胡春卉,尹瑞兴,等.广西罗城县少数民族和汉族人群血清EB病毒感染情况分析[J].重庆医学,2012,41(14):1404-6.]
    [10] Zhang CQ,Song YF,Zhu XJ,et al.Analysis of EB virus antibody level in population of Fuzhou area[J].Zhongguo Wei Sheng Jian Yan Za Zhi, 2012,22(4):864-5.[张臣青,宋艳芳,祝先进,等.福州地区部分人群EB 病毒VCA-IgA 抗体水平的调查分析[J].中国卫生检验杂志,2012,22(4):864-5. ]
    • 相关文章
    • 施引文献
    • 资源附件(0)
计量
  • 文章访问数:  2478
  • HTML全文浏览量:  25
  • PDF下载量:  386
  • 被引次数: 0
出版历程
  • 收稿日期:  2012-09-23
  • 修回日期:  2012-11-20
  • 刊出日期:  2013-04-24

目录

摘要
HTML全文
    参考文献(10)
    相关文章
    施引文献
    资源附件(0)

    /

    返回文章
    返回

    下载中心

    友情链接

    联系我们

    地址：武汉市洪山区卓刀泉南路116号（430079）

    电话：027－87670126

    Email：zlfzyjzz@vip.163.com

    This work is licensed under a Creative Commons Attribution 3.0 License.

    邮件订阅 RSS
    总访问量：12056696 今日访问：20375

    版权所有 © 《肿瘤防治研究》编辑部 鄂公网安备 42011102005013号 鄂ICP备2022015867号

    本系统由北京仁和汇智信息技术有限公司开发  百度统计

    x 关闭 永久关闭