Abstract: Objective To evaluate the relationship between ages and the Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG values and positive rate of the Epstein-Barr virus (EBV) antibodies in Zhuang NPC patients at different ages in west Guangxi. Methods Serum samples obtained from140 patients with nasopharyngeal carcinoma (NPC) and from 280 healthy people were examined for the EBV antibodies including Rta/IgG,VCA/IgA,VCA/IgG and Zta/IgG by the enzyme-linked immnunosorbent assay (ELISA).The positive rates and antibody levels of the NPC patients at different ages were analyzed statistically. Results Significant difference in VCA/IgA values and positive rates was found in the NPC patients at different ages (P<0.05).There was significant difference in the positive rates of Rta/IgG and VCA/IgA between the patients and healthy people above 50 years old (P<0.05).But no significant difference in the positive rates of Zta/IgG was found between them (P>0.05). Conclusion The serum levels and positive rates of EB virus antibodies (Rta/IgG,VCA/IgA and Zta/IgG) fluctuate with different ages. Different positive critical values of EBV antibodies for respective age groups should be set to facilitate NPC diagnosis and high-risk screening.