Abstract: Objective To investigate the effects of methylation inhibitor 5-Aza-2-deoxycyt(5-Aza-CdR) on the invasion and migration of pancreatic carcinoma cell line PANC-1,and to explore its possible mechanisms. Methods PANC-1 cell line was treated by 5-Aza-CdR of different concentrations.Wound scratch assay and transwell chamber were used to detect the ability of the invation and migration of PANC-1 cells.The expression of MMP-2 was detected by immunofluorescent staining. Results After being treated by 5-Aza-CdR,compared with the control group, the ability of migration and invasion was reduced obviously(P<0.05). Immunocytochemical analysis indicated that the expression of MMP-2 was down-regulated after being treated by 5-Aza-CdR　(P<0.05). Conclusion 5-Aza-CdR can inhibit the invasive ability of PANC-1 cells, and its mechanism might be reducing the invasion and migration of the cells by inhibiting the expression of MMP-2.