Abstract: Objective To study the relationship between aberrant methylation and expression of runx3 gene and susceptibility of primary hepatocellular carcinoma．Methods Research literature of cancer suppressor gene runx3 and primary hepatocellular carcinoma susceptibility home and abroad. ResultsA total of 841 cases and 800 controls from 14 studies were included．Expression of runx3 gene was different in hepatocellular carcinoma and its para cancer tissue,OR=0.10（95％CI：0.04-0.23)，so was methylation of runx3 OR=21.62（95％CI：8.06-57.98). Conclusion The positive expression ratio of runx3 gene in the HCC tissue was significantly lower than those in para-cancer tissue.And methylation of runx3 gene was significantly higher than those in para-cancer tissue. Methylation of cancer suppressor gene runx3 will lead to abnormal expression of hepatoma carcinoma cells.Occurrence and development of hepatoma carcinoma has correlation to abnormal expression and methy lation of runx3.Detection of expression missing and abnormal methylation of runx3 could be the early auxiliary diagnosis means for hepatoma carcinoma patients,which could also become the molecular therapy target.