食管鳞癌3p14.2-14.1上缺失区的检测

Detection of LOH on 3 p14.2-14.1 in Esophageal Squamous Cell Carcinoma

摘要: 目的寻找食管鳞癌（ESCC）的3号染色体短臂（3p）上微小重叠缺失区，为筛选与ESCC发病相关的肿瘤抑制基因提供实验依据。方法采用微卫星分析方法检测食管鳞癌及其配对的癌旁正常组织杂合性丢失情况。结果食管癌中5个分布在3p21.1-p13区域的微卫星标志出现高频改变，连锁分析发现这一区域存在一个微小重叠缺失区，该区分布在3p14.2-p14.1，介于D3S2452、D3S3040和D3S1766之间，大小约283 kb。结论在3p14.2-p14.1区域可能存在一些新的肿瘤抑制基因，可进一步在此区域作ESCC相关基因研究。

Abstract: Objective To search for small overlap missing area on the third chromosome short arm in esophageal squamous cell carcinoma (ESCC) so as to provide the experimental basis for screening tumor ESCC related suppressor genes. Methods Microsatellite analysis method was used to detect the heterozygosity lost (LOH) of ESCC and normal tissue adjacent to the cancer. Results Five microsatellite markers distributing on 3 p21.1-p13 area showed high frequency change,chain analysis indicated that there was a small overlap missing area in 3 p14.2-p14.1,identified as the markers D3S2452,D3S3040 and D3S1766,encompassing approximately 283 kb long. Conclusion There might be some new tumor suppressor genes in 3p14.2-p14.1 area.It could be valuable to further investigation for the related genes of ESCC in the area.