Effect of Rapamycin on Proliferation of Human Cervical Carcinoma HeLa Cell and Expression of HIF-1α and VEGF
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摘要: 目的 探讨不同浓度雷帕霉素对宫颈癌HeLa细胞生长的抑制作用及对HIF-1α、VEGF表达的影响。方法采用四甲基偶氮唑盐（MTT）比色法检测分别以不同浓度雷帕霉素处理的体外培养人宫颈癌HeLa细胞的抑制率，采用反转录-聚合酶链反应（RT-PCR）、Western blot 法检测各组细胞HIF-1α、VEGF mRNA和蛋白的表达。结果不同浓度雷帕霉素对宫颈癌HeLa细胞的生长有明显的抑制作用，并呈剂量依赖性；随雷帕霉素浓度增加细胞内HIF-1α、VEGF mRNA和蛋白的表达均呈下调趋势。结论雷帕霉素对宫颈癌HeLa细胞的生长有抑制作用并呈剂量依赖性，雷帕霉素具有明显下调HIF-1α、VEGF mRNA和蛋白表达的作用。Abstract: Objective To explore the effects of different concentrations of rapamycin on growth inhibition and influence on HIF-1 alpha, VEGF expression in HeLa cervical cancer cells. MethodsUsing four methylazo thiazole blue (MTT) colorimetric method to detect the inhibition ratio of in vitro human cerical cancer HeLa cells processed with different concentraions of rapamycin, the reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to detect mRNA and protein levels of HIF-1 alpha and VEGF. Results Different concentrations of rapamycin inhibited HeLa cervical cancer cells growth signnificantly, with a dose-dependent mode; with increasing concentration of rapamycin, both mRNA and protein levels of HIF-1 alpha, VEGF were reduced. Conclusion Rapamycin inhibited HeLa cervical cancer cells growth with a dose-dependent mode, and rapamycin obviously down-regulated HIF-1 alpha, VEGF at both mRNA and protein levels.
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