Expression and Clinical Signifiance of STAT-3 and Enolase-1 in Breast Cancer
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摘要: 目的 探讨乳腺癌组织中信号转导及转录活化因子3（STAT-3）和糖酵解酶烯醇化酶（Enolase-1）的表达与雌孕激素受体、淋巴结转移、临床分期、病理分级等的关系。方法免疫组织化学SP法检测126例乳腺癌和26例乳腺良性病变组织中STAT-3与 Enolase-1的表达情况，并分析他们与临床病理参数之间的关系。结果乳腺癌组织中STAT-3和 Enolase-1的阳性表达率分别为82.5％和77.8％,明显高于乳腺良性病变组织（11.5％和7.7％），差异均有统计学意义（χ2=42.416,P<0.05;χ2=57.211, P<0.05）；在有淋巴结转移的乳腺癌组织中阳性表达率分别为85.7％和90.5％,高于无淋巴结转移组，差异均有统计学意义（χ2=9.184，P<0.05；χ2=11.014，P<0.05）。相关分析表明STAT-3和 Enolase-1的表达呈正相关。在雌激素受体阳性（ER+）和/或孕激素受体阳性（PR+）或表皮生长因子受体2阳性(HER-2+)乳腺癌组织中，有淋巴结转移组中STAT-3和Enolase-1的表达水平均明显高于无淋巴结转移组，差异均有统计学意义（P<0.05）。结论STAT-3与Enolase-1的表达与乳腺癌的发生发展及侵袭转移相关，且二者有协同作用（r=0.379，P<0.05）。在ER+和(或)PR+或HER-2+乳腺癌组织中，STAT-3和Enolase-1的高表达与淋巴结转移相关。在高表达STAT-3的乳腺癌组织中Enolase-1表达也较高，其联合检测可作为判断乳腺癌恶性程度、评价预后及指导治疗的一项评估指标。Abstract: Objective To investigate the expression of STAT-3 and Enolase-1 in breast cancer and its relationship with Estrogen receptor status,Progesterone receptor status,lymph node metastasis,clinical stages and histological grading. Methods The expression of STAT-3 and Enolase-1 were examined by immunohistochemical streptavid-peroxidase(S-P)assay in 126 cases of breast cancer and 26 cases of benign breast disease.And the significance of its expression was analyzed with clinicopathologic parameters. Results The expression rates of STAT-3(77.8％)and Enolase-1(82.5％)in breast cancer were significantly higher than that in benign breast disease（11.5％ vs.7.7％）and the expression of two proteins were positively correlated(χ2=42.416,P<0.05;χ2=57.211，P<0.05).The expression of STAT-3 and Enolase-1 in breast cancer has no significant difference in age,tumor size,histologic grading and clinical stages.Epression of STAT-3(85.7％)and Enolase-1(90.5％)in breast cancer with metastasis is significantly higher than that without metastasis （χ2=9.184,P<0.05;χ2=11.014,P<0.05）.There was a significant correlation between the high expression of STAT-3 and axillary lymphatic metastasis in comparisons between positive estrogen and/or progesterone receptor expression and Her-2 positive expression（P<0.05）.And there was a significant correlation between the high expression of Enolase-1 and axillary lymphatic metastasis in comparisons with positive estrogen or positive progesterone receptor expression and Her-2 positive expression（P<0.05）. Conclusion Both STAT-3 and Enolase-1 may be involved in the tumorgenesis development and metastasis of breast cancer.The high expression of STAT-3 and Enolase-1 in estrogen receptor or/and progesterone receptor or Her-2 positive breast cancer was associated with a high possibility of lymph node metastasis.The detection of STAT-3 and Enolase-1 may be helpful in the diagnosis and prognosis of breast cancer.
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Key words:
- Breast cancer /
- Lymph node metastasis /
- STAT-3 /
- Enolase-1
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