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小鼠结肠癌肝转移前肝脏Th1/Th2细胞因子的变化及与门静脉IL-10、TGF-β1的相关性

檀谊洪, 邱万寿, 杜国能, 肖玉根, 陈图峰, 魏 波

檀谊洪, 邱万寿, 杜国能, 肖玉根, 陈图峰, 魏 波. 小鼠结肠癌肝转移前肝脏Th1/Th2细胞因子的变化及与门静脉IL-10、TGF-β1的相关性[J]. 肿瘤防治研究, 2012, 39(10): 1166-1169. DOI: 10.3971/j.issn.1000-8578.2012.10.002
引用本文: 檀谊洪, 邱万寿, 杜国能, 肖玉根, 陈图峰, 魏 波. 小鼠结肠癌肝转移前肝脏Th1/Th2细胞因子的变化及与门静脉IL-10、TGF-β1的相关性[J]. 肿瘤防治研究, 2012, 39(10): 1166-1169. DOI: 10.3971/j.issn.1000-8578.2012.10.002
Tan Yihong, Qiu Wanshou, Du Guoneng, Xiao Yugen, Chen Tufeng, Wei Bo. Shift of Hepatic Th1/Th2 Cytokines and Its Correlation with IL-10 and TGF-β1 in Portal Vein of Colon Tumor-bearing Mice Previous to Liver Metastasis[J]. Cancer Research on Prevention and Treatment, 2012, 39(10): 1166-1169. DOI: 10.3971/j.issn.1000-8578.2012.10.002
Citation: Tan Yihong, Qiu Wanshou, Du Guoneng, Xiao Yugen, Chen Tufeng, Wei Bo. Shift of Hepatic Th1/Th2 Cytokines and Its Correlation with IL-10 and TGF-β1 in Portal Vein of Colon Tumor-bearing Mice Previous to Liver Metastasis[J]. Cancer Research on Prevention and Treatment, 2012, 39(10): 1166-1169. DOI: 10.3971/j.issn.1000-8578.2012.10.002

小鼠结肠癌肝转移前肝脏Th1/Th2细胞因子的变化及与门静脉IL-10、TGF-β1的相关性

基金项目: 广东省科技计划项目基金资助项目(2008B 030301128)
详细信息
    作者简介:

    檀谊洪(1973-),男,硕士,主治医师,主要从事结直肠肿瘤和甲状腺外科的研究

    通讯作者:

    邱万寿,E-mail:wsqiu-d@163.com

  • 中图分类号: R735.3+5

Shift of Hepatic Th1/Th2 Cytokines and Its Correlation with IL-10 and TGF-β1 in Portal Vein of Colon Tumor-bearing Mice Previous to Liver Metastasis

  • 摘要: 目的 探讨在小鼠结肠癌肝转移发生之前,门静脉中的肿瘤可溶性产物IL-10、TGF-β1水平与肝脏Th1/Th2细胞因子的变化情况及其相关性。方法20只BALB/c小鼠随机分为两组:盲肠肿瘤块(CT26细胞)种植法建立原位结肠癌肝转移模型为试验组,对照组为假手术组。2周后提取门静脉血及肝脏标本,ELISA方法检测门静脉血IL-10、TGF-β1及肝脏组织IFN-γ、IL-10浓度,比较两组间差异并计算相关性。结果实验组小鼠肝脏Th1/Th2(IFN-γ/IL-10)比值较对照组降低,且与门静脉IL-10和TGF-β1的浓度呈显著负相关。结论小鼠结肠肿瘤可释放IL-10、TGF-β1入门静脉,导致肝脏Th1/Th2样细胞因子(IFN-γ/IL-10)出现漂移现象,可能会促进肝转移的发生。

     

    Abstract: Objective To explore the correlation between the level of IL-10 and TGF-β1 in portal vein and the ratio of Thl/Th2 like cytokines in liver previous to liver metastasis in mice models of metastasis. Methods Twenty mice were randomly assigned into control group and experimental group.Established the orthotopic model of liver metastasis by implanting tumor block into the cecal wall in BALB/c mice as experimental group,and the other ten were accepted the sham operation.After two weeks,all mice were killed and blood samples in portal veins and liver samples were collected for ELISA testing of IL-10,TGF-β1 and IFN-γ.The t-test was used to compare the differences between two groups,and Bivariate correlation analysis was used to analyze the correlation. Results Compared to the control group,the ratio of Th1/Th2(IFN-γ/IL-10) in livers of experimental mice was decreased significantly,which showed a significantly negative correlation with serum levels of IL-10 and TGF-β1 in portal vein. Conclusion Tumors of mouse colon can produce and release IL-10 and TGF-β1 into portal vein,which can flow to liver and lead to the shift of Th1/Th2 like cytokines to promote the liver metastasis.

     

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出版历程
  • 收稿日期:  2011-11-06
  • 修回日期:  2012-02-13
  • 刊出日期:  2012-10-24

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