Effect of Mitomycin on DNA Damage and Proliferation of Gastric Cancer Cell in vitro
-
摘要: 目的 探讨丝裂霉素对体外胃癌细胞DNA损伤和增殖的影响。方法选用不同浓度丝裂霉素处理体外培养的胃癌细胞SGC-7901，利用免疫荧光和Western blot技术，分别检测γH2AX的焦点形成和蛋白水平变化；使用MTT法检测SGC-7901细胞的增殖情况。结果丝裂霉素的作用浓度和作用时间存在交互效应，除400 μg/ml外，平均γH2AX焦点数和焦点细胞率呈逐渐增加趋势（P<0.05）；400 μg/ml 时，6 h组平均γH2AX焦点数和焦点细胞率低于4 h组，差异有统计学意义（P<0.05）。随丝裂霉素作用时间延长，γH2AX蛋白水平呈先升后降趋势，差异有统计学意义（P<0.05）。MTT结果显示，丝裂霉素对SGC-7901细胞的增殖有明显抑制作用（P<0.05）。结论γH2AX可敏感反应丝裂霉素对胃癌细胞DNA的损伤，丝裂霉素对体外胃癌细胞增殖抑制明显。
-
关键词:
- 丝裂霉素 /
- 胃癌SGC-7901细胞 /
- γH2AX /
- DNA双链断裂 /
- 增殖
Abstract: Objective To study mitomycin-induced DNA damage and proliferation inhibition of gastric cancer cells by detection of γH2AX in vitro. Methods Mitomycin with different concentrations was used to incubate gastric cancer cell line SGC-7901 cells in vitro.Focus formation and protein levels of γ H2AX were detected by immunofluorescence and western-blotting techniques, respectively, MTT assay was used to detect the proliferation of gastric cancer cell. Results The mitomycin existed concentration and time interaction effect. Except 400 μg/ml, the average number of γH2AX foci and focus cell rate were gradually increased (P<0.05). At 400 μg/ml, in 6 h group average number of γH2AX foci and focus of cell rate were less than those in 4 h group (P<0.05). With time of mitomycin treatment prolonged,γH2AX protein levels were increased and then decreased (P<0.05). MMC significantly inhibited the proliferation of SGC-7901 (P<0.05). Conclusion γH2AX was a sensitive marker to predict DNA damage and proliferation inhibition of gastric cancer cell induced by Mitomycin C in vitro.-
Key words:
- Mitomycin /
- Gastric cancer SGC-7901 cells /
- γH2AX /
- DNA double-strand break /
- Proliferation
-
-
[1] Wu YJ,Tang Y,Zhang Y,et al.Clinicopathological significance of cofilin1 in gastric cancer tissues[J].Zhong Liu Fang Zhi Yan Jiu,2012,39(3):295-98.[吴勇军,唐仪,张漾,等.cofilin1在胃癌组织中表达的临床病理意义[J].肿瘤防治研究,2012,39(3):295-98.] [2] Bai JJ,Jiang YN,Sun L,et al.Effects of mitomycin on ultrastractrure and expression of TOPO Ⅱ α in humangastric cancer cells [J].Hebei Bei Fang Xue Yuan Xue Bao(Yi Xue Ban),2008,25(4):22-4.[白金君,蒋亚楠,孙黎,等.丝裂霉素对人胃癌细胞超微结构和拓朴异构酶表达的影响[J].河北北方学院学报（医学版）,2008,25(4):22-4.] [3] Rothkamm K,Horn S.Gamma-H2AX as protein biomarker for radiation exposure [J].Ann Ist Super Sanita,2009,45(3):265-71. [4] Parkin DM,Bray F,Ferlay J,et al.Global cancer statistics,2002 [J].CA Cancer J Clin,2005,55(2):74-108. [5] Mao DL,Ye W,Li FQ,et al.The relationship between spatial distribution of gastric cancer and geographicalenvironment [J].Zhejiang Yu Fang Yi Xue,2011,23(6):5-8.[毛德玲,叶玮,李凤全,等.胃癌空间分布与地理环境关系的研究[J].浙江预防医学,2011,23(6):5-8.] [6] Redon CE,Dickey JS,Bonner WM,et al.γ-H2AX as a biomarker of DNA damage induced by ionizing radiation inhuman peripheral blood lymphocytes and artificial skin [J].Adv Space Res,2009,43(8):1171-8. [7] Zou P,Yang XL,Chen X.The effect of bleomycin on DNA damage and H2AX interacting proteins in hepatocellularcarcinoma(HCC) cells [J].Fudan Xue Bao(Zi Ran Ke Xue Ban),2011,50(2):172-7.[邹鹏,杨晓丽,陈先.博来霉素对肝癌细胞DNA损伤以及H2AX相互作用蛋白的影响[J].复旦学报(自然科学版),2011,50(2):172-7.] [8] Ai J,He LX,Liang SY.Inhibitiory effect of antitumor drugs on growth of gastric cancer cell in vitro[J].Hebei Yi Ke Da Xue Xue Bao,2002,23(1):19-21.[艾军,何兰欣,梁锁源,等.抗癌药物对体外胃癌细胞生长的抑制作用[J].河北医科大学学报,2002,23(1):19-21.]
计量
- 文章访问数: 2138
- HTML全文浏览量: 30
- PDF下载量: 543
下载:
