Relationship of Cancer Stem Cells with Clinical Pathologic Factors,survivin and Ki67 in Colorectal Carcinoma
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摘要: 目的 探讨肿瘤干细胞标志物CD133在结直肠癌组织中的表达,及其与临床病理因素、survivin和Ki67的关系。方法应用免疫组织化学方法检测60例手术切除的结直肠癌和30例癌旁组织及12例正常组织中CD133的表达、癌组织中survivin和Ki67的表达,对各指标之间的相关关系及其与性别、年龄、肿瘤的部位和大小、病理分级、侵袭深度、淋巴结转移、pTNM分期之间的关系进行分析。结果结直肠癌组织中CD133阳性表达率为50％(30/60),显著高于癌旁及正常组织(P<0.01);CD133的表达与淋巴结转移和pTNM分期密切相关(P<0.01),与病理分级存在相关性(P<0.05),与其他临床病理因素均无关;survivin的表达仅与肿瘤的病理分级相关(P<0.05),Ki67与各项临床病理参数均无相关性;CD133与survivin、Ki67的表达呈正相关(P<0.05、P<0.01);survivin与Ki67的表达呈正相关(P<0.01)。结论结直肠癌组织中存在CD133+肿瘤细胞,肿瘤干细胞在结直肠癌的发生、发展中起重要作用,检测肿瘤干细胞标志物CD133的表达可作为一种判断肿瘤的恶性程度和预后的重要指标。Abstract: Objective To explore the expression of cancer stem cell marker CD133 and to elucidate its relationship with the clinicopathological factors,survivin and Ki67 in colorectal cancer tissues. Methods Expressions of CD133,survivin and Ki67 were detected by immunohistochemical method in 60 cases of colorectal carcinoma,para-cancerous and normal colorectal tissues.The correlations between the various indicators as well as the relationship between these indicators and clinicopathological factors including gender,age,site of primary mass,tumor size,histological grade,lymph node metastasis,invasive depth and pTNM stage were analyzed. Results The positive rate of CD133 was 50% in the 60 cases of colorectal cancer,which was significantly higher than that in para-cancerous and normal colorectal tissues (P<0.01).Expression of CD133 was significantly associated with lymph node metastasis and pTNM stages (P<0.01),and correlated with histological grades statistically(P<0.05),but not related to other clinicopathological parameters.Expression of survivin was only related to histological grades of tumor(P<0.05).No correlation between Ki67 and any clinicopathological parameters.Expression of CD133 was positively correlated with the expressions of survivin and Ki67(P<0.05 and P<0.01),respectively.Expression of survivin was also positively correlated with the expression of Ki67(P<0.01). Conclusion CD133+ tumor cells exist in colorectal cancer tissues.Cancer stem cells play an important role in the carcinogenesis and progreis of colorectal cancer.The expression of cancer stem cell marker CD133 may be useful for evaluating the malignancy and prognosis of colorectal carcinoma.
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Key words:
- Colorectal cancer /
- CD133 /
- Cancer stem cells
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