Inhibition of G31P:Chemokine Receptor CXCR1/CXCR2 Antagonist,in Angiogenesis of Human Prostate Cancer Cells in vivo
-
摘要: 目的 探讨G31P（CXCR1/CXCR2受体拮抗剂）对人前列腺癌PC-3细胞的体内血管新生的抑制作用。方法建立体内绿色荧光蛋白(GFP)标记的人雄激素非依赖性前列腺癌细胞PC-3的裸鼠原位移植瘤模型，观察G31P对裸鼠前列腺原位移植瘤血管新生的影响。结果与对照组（1.26±0.46）相比，G31P处理组明显抑制前列腺肿瘤的血管新生（0.49±0.12，P<0.05)，与对照组相比，G31P处理组VEGF （P<0.01）和NF-κB（P<0.01）的表达具有统计学意义(免疫组织化学法)。结论在裸鼠原位移植瘤模型中G31P对人雄激素非依赖性前列腺癌的血管新生有明显抑制作用。Abstract: Objective To investigate the inhibition of G31P on the angiogenesis of the prostate cancer PC-3 cell in vivo. Methods The effect of G31P on angiogenesis of human prostate tumor of nude mice were observed in nude mice by building a human androgen-independent prostate cancer PC-3 (GFP-labeled) orthotopic transplantation tumor cells model. Results The tumor angiogenesis of G31P treated group （1.26±0.46）was significantly reduced （0.49±0.12，P<0.05) compared with the control group.VEGF（P<0.01）and NF-κB（P<0.01）expression of G31P treated groupwas significantly reduced (immunohistochemistry) compared with the control group. Conclusion G31P could inhibit the angiogenesis of the prostate cancer PC-3 cell in vivo.
-
Key words:
- G31P /
- Prostate cancer /
- Angiogenesis /
- CXCR1 /
- CXCR2
-
-
[1] Vicari AP,Caux C.Chemokines in cancer[J].Cytokine Growth Factor Rev,2002,13(2):143-54. [2] O′Hayer KM,Brady DC,Counter CM.ELR+ CXC chemokines and oncogenic Ras-mediated tumorigenesis[J].Carcinogenesis,2009,30 (11):1841-7. [3] Wang J.Progress of the application of vascular endothelial growth factor and its receptor in anti-tumortherapy[J].Zhongguo Yi Yao Gong Ye Za Zhi,2007,38(5):381-6.[王娟.血管内皮生长因子及其受体在抗肿瘤治疗应用的研究进展[J].中国医药工业杂志,2007,38(5):381-6.] [4] Gaurnier-Hausser A,Tuszynski GP.The immunomodulatory role of angiocidin,a novel angiogenesis inhibitor [J].Curr Pharm Des,2009,15(17),1937-48. [5] Heidemann J,Ogawa H,Dwinell MB,et al.Angiogeneic effects of interleukin 8 (CXCL8) in humanintestinalmicrovascular endothelial calls are mediated by CXCR2 [J].J Biol Chem,2003,278 (10):8508-15. [6] Addison CL,Daniel TO,Burdick MD,et al.The CXC chemokine receptor 2,CXCR2,is the putative receptor for ELR+CXC chemokine-induced angiogenic activity [J].J Immunol,2000,165 (9):5269-77.
计量
- 文章访问数: 2142
- HTML全文浏览量: 16
- PDF下载量: 665
下载:
