Abstract: Objective To evaluate the growth inhibition of gastric cancer cells by Oxaliplatin (L-OHP) and Cisplatin (CDDP), and the different mechanism of the two platinum compounds. Methods Gastric cancer cells (BGC-823 and SGC-7901) were treated with different inhibiting concentrations (IC10, IC30 or IC50) of L-OHP or CDDP for 24, 48 and 72 hours, respectively. The proliferation inhibition ratios of gastric cancer cells were evaluated by MTT assay, and the expressions of MDR1 mRNA were detected by RT-PCR. Apoptosis and cell migration ability were evaluated by FCM and Cell Scratch Test respectively under IC30 for two compounds. Results The proliferation of BGC-823 and SGC-7901 was inhibited in both dose-and time-dependent manners by the CDDP or L-OHP, especially for L-OHP, dose-dependent effect was more significant. MDR1 was up-regulated in CDDP or L-OHP group. After the treatment of two platinum compounds, the apoptosis level of BGC-823 was significanctly higher than that of SGC-7901. Meanwhile, the apoptosis level induced by L-OHP was significanctly higher than that by CDDP. After the treatment of two platinum compounds, BGC-823 cells had the slower and shorter immigration than SGC-7901. Meanwhile, the faster speed and longer distance of immigration was observed in CDDP than that in L-OHP. Conclusion L-OHP was more efficient than CDDP in inducing apoptosis and suppresseing cell migration. The two platinum compounds could induce the expression of MDR1, which might be a potential resistance mechanism of platinum compounds in gastric cancer.