Abstract: Objective To explore the relationship of CD34,8-OHdG and Ki67 expression with clinical characteristics and post-operational survival hepatitis B virus related hepatocellular carcinoma. Methods Follow-up was performed every three months for 5 years.The expression level of 8-OHdG,CD34 and Ki67 were quantified by immunohistochemical staining. Results The frequency of positive staining for 8-OHdG,CD34 and Ki67 in HBV-HCC tissues was 100%,100% and 96.74%,respectively.Furthermore,the expression level of CD34 was higher in HBV-HCC tissue than that in normal tissue.The expression of CD34,8-OHdG and Ki67 showed a positive correlation each other.Multivariate Cox regression analysis indicated that CD34,8-OHdG and Ki67 were the independent predictors of post-operational survival for HBV-HCC patients. Conclusion CD34,8-OHdG and Ki67 is independent predictors for the prognosis of HCC.