Abstract: Objective To explore the mechanism underlying the inhibiting effects of miR-126 on the invasion of human brain glioma cells. Methods miR-126 was synthesized and transfected into the U87 malignant glioma cells by liposome.The relative expression of MMP-2 and MMP-9 gene was quantified by RT-PCR，the expression of MMP-2 and MMP-9 protein was detected by Western blot，and cell invasion was assessed by Transwell chamber assay. Results miR-126 inhibited the activity of MMP-2 and the invasion of human U87 glioma cells. Conclusion The chemically synthesized miR-126 plays an important role in cell invasion of human brain glioma cells，which may be a promising target for glioma gene therapy.