腺病毒介导的IL-24对人大细胞肺癌NCI-H460细胞的体外抑制效应
Interleukin 24 Inhibits Growth and Induces Apoptosis of Human Large Cell Lung Cancer Cell Line NCI- H460 in vitro
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摘要: 目的 研究腺病毒介导的IL-24基因表达对NCI-H460肺癌细胞抑癌增效作用及分子机制。方法将Ad-IL-24重组腺病毒感染NCI-H460细胞。以Western blot法鉴定IL-24基因在NCI-H460细胞中的表达；MTT法检测重组腺病毒对NCI-H460细胞的生长抑制作用；经Annexin-V-PE/7-AAD染色后流式细胞术（FCM）检测细胞凋亡率变化；RT-PCR检测NCI-H460细胞中bax、caspase-3、bcl-2、survivin等因子的表达。结果腺病毒介导的IL-24基因在NCI-H460细胞中能够有效表达；Ad-IL-24组对NCI-H460细胞的生长具有明显的抑制作用， Ad-IL-24能够上调bax、caspase-3等因子的表达，下调bcl-2、survivin等因子的表达，诱导细胞凋亡。结论腺病毒介导的IL-24 基因在体外可明显抑制人肺癌细胞NCI-H460的生长，诱导其凋亡，其分子机制可能与上调bax、caspase-3等促凋亡因子的表达，下调bcl-2、survivin等凋亡抑制因子的表达有关。Abstract: Objective To study the enhanced anticancer effect and its mechanism by Ad-polyA-promoer-IL-24（Ad-IL-24） for NCI-H460 cells. Methods Transfected into NCI-H460 cells with the different recombinant adenovirus，the expression of IL-24 was identified by Western blot.The influence of the cell growth was tested by MTT essay.The cell apoptotic effect was evaluated by flow cytometry(FCM)，which were dyed by Annexin-V-PE /7-AAD.The expressions of cytokines including bax，caspase-3，bcl-2 and survivin were explored by RT-PCR. Results Western blot proved that IL-24 was effectively transcribed in NCI-H460 cells.Ad-IL-24 can induce apoptosis by up-regulating bax，caspase-3 expression while down-regulating bcl-2 and survivin expression．Cell growth was obviously inhibited by the recombinant adenovirus. Conclusion It was proved that there was enhanced anticancer effect by Ad-IL-24.The mechanism may be related to the up-regulations of bax，caspase-3 and the down-regulations of bcl-2，survivin.
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