胃肠肿瘤患者手术前后外周血survivin mRNA定量检测的临床意义
Clinical Significance of Quantitative Detection of survivin mRNA in Peripheral Blood of Gastrointestinal Cancer Patients before and after Operation
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摘要: 目的研究胃肠肿瘤患者手术前后外周血survivin mRNA表达量的变化 ，分析其与复发转移的关系。方法采用实时荧光定量 RT - PCR检测40例胃肠肿瘤患者手术前后外周血survivin mRNA表达量,并进行6月的随访；同时以10例患胆石症或阑尾炎的非癌手术病人作为对照。结果40例胃肠肿瘤患者，手术前后外周血survivin mRNA阳性率分别为87.5%和45%，两者比较差异有统计学意义(P<0.01）；5例术前未检出survivin mRNA表达的患者术后均未检出；35例survivin mRNA阳性表达的患者中术后17例未检出，12例survivin mRNA水平明显低于术前,6例survivin mRNA水平增高；6例术后survivin mRNA水平增高的患者在随访6月内有4例出现明显的肝转移。结论检测手术前后外周血survivin mRNA水平的变化，可作为观察胃肠肿瘤术后复发转移的一种非侵入性、可测量的客观指标。Abstract: ObjectiveTo study the change of survivin mRNA expression in peripheral blood of gastrointestinal cancers patients before and after operation and explore its feasibility of predicting gastrointestinal cancers recurrence and metastasis. Methods survivin mRNA were detected by real time quantitative RT-PCR in peripheral blood of 40 gastrointestinal cancers patients before and after operation.The follow-up lasted 6 months after operation.Ten resected patients with appendicitis or gall-stone as controls. Results Post-operative survivin expression in 18 (45%) was signifcantly lower than pre-operative expression in 35(87.5%) of 40 cancer patients.Post-operative survivin levels were all non-detectable in 5 cancer patients non-detectable pre-operatively.Post-operative survivin levels were non-detectable in 17 cancer patients and were signifcantly lower than pre-operative levels in 12 cancer patients and were higher than pre-operative levels in 6 cancer patients of 35 resected patients.In 6 months liver metastasis were signifcantly detected in 4 patients of 6 patients that survivin detected higher than pre-operative. Conclusion The change of survivin mRNA levels in peripheral blood before and after operation was a non-invasive and detectable impersonal data to predict cancer recurrence and metastasis.
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