Abstract: ObjectiveTo research the mechanism of effects of HIF-1α (YC-1) on implanted human primary hepatic carcinoma in nude mice. MethodsA total of 16 male nude mice were inoculated subcutaneously with 1 million SMMC-7721 cells. Once the tumor grew to (100~150)mm3, the mice were divided randomly into two groups and injected with YC-1 or DMSO respectively. Tumor size and weight were measured. Levels of HIF-1α and VEGF expressions in tumor tissue were detected by RT-PCR, Westetn blot or immunohistochemical respectively. ResultsTumors grew rapidly in the control group than those in the YC-1 treatment group. In YC-1 group therapy resulted in low expression of HIF-1α and VEGF than those in control groups(P<0.05). ConclusionYC-1 therapy was a promising approach to treat human liver cancer by suppression of HIF-1α and VEGF expression, which might contribute to inhibit tumor growth and angiogenesis.