Abstract: Objective To investigate the relation between the classification of MUC2 and CDX2 in colorectal adenocarcinoma and its clinical pathological parameters. Methods The expression of MUC2 and CDX2 was detected by immunohistochemistry in 30 specimens of colorectal adenoma, 60 specimens of colorectal adenocarcinoma and 30 specimens of normal colorectal tissues. Results The positive rates of MUC2 in normal colorectal mucosa,colorectal adenoma and colorectal adenocarcinoma were 100%,93.3%,58.3% respectively. CDX2 were 90.0%,76.7%,31.7%. Colorectal adenocarcinoma could be classified into four phenotypes: MUC2+CDX2+, MUC2+CDX2-,MUC2-CDX2+,MUC2-CDX2-.MUC2+CDX2+ showed significant correlation with differentiation, depth of invasion, Dukes staging and survival period. MUC2+CDX2- showed significant correlation with lymph node metastasis. MUC2- CDX2+ showed no significance in relation to all the clinical pathological parameters. MUC2-CDX2- showed significant correlation with differentiation, depth of invasion, lymph node metastasis,Dukes staging and survival period. Conclusion The dawn-regulation of MUC2 and CDX2 may participate in the pathogenesis of colorectal adenocarcinoma. Classification of colorectal adenocarcinoma is relevant to the genesis、progression、invasion and metastasis of colorectal adenocarcinoma., which can be used to predict the patients' prognosis.