rhBMP-2抑制胶质瘤细胞方式的初步探讨

Study on the action mode of morphogenetic PROTEIN-2 inhibiting glioma cells

摘要: 目的　初步探讨BMP 2抑制胶质瘤细胞的作用方式。方法　在培养的人脑胶质瘤细胞系SHG 4 4中加入rhBMP 2, 测定生长曲线, 应用流式细胞仪法分析细胞周期, 用FITC标记的AnnexinV染色法分析凋亡和坏死的比例。结果　经rhBMP 2作用的SHG 4 4细胞G1期所占比例明显升高 (P <0 .0 1) ;10 μg/ml组中, 完整细胞 (PI /FITC )比例 (35 .6 % )明显低于 5 μg/ml组 (94 .3% )和对照组 (96 .9% ) (P <0 .0 1), 坏死细胞(PI+ /FITC + )比例 (5 8.0 % )明显高于 5 μg/ml组 (3.1% )和对照组 (0 .5 % ) (P <0 .0 1)。结论　在rhBMP - 2抑制胶质瘤细胞SHG 4 4增殖中, 诱导其坏死是主要作用, 其中也有凋亡作用的参与 ;AnnexinV染色法较流式细胞仪法可更加准确的区分正常细胞, 早、晚期凋亡细胞和坏死细胞。

Abstract: Objective To investigate the action mode of bone morphogenetic protein-2 (BMP-2) inhibiting glioma cell. Methods We incubated cultured human SHG-44 glioma cell with recombinated bone morphogenetic protein-2 (rhBMP-2) protein. Cell growth curve was characterized. Cell cycle and Annexin V staining were detected by flow cytometry for studing the action mode of rhBMP-2. Results The DNA content of SHG44 increased in G1cycle, and decreased in S cycle obviously ( P <0.01)in groups incubated with rhBM...