脂质体-c-erbB-2反义寡核苷酸转染对人卵巢癌裸鼠皮下移植瘤形成的抑制作用研究
Inhibitory effects of liposome-c-erbB-2 antisense oligodeoxynucleotides transfection on the human ovarian carcinoma transplanted subcutaneously in nude mice
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摘要: 目的 探讨经脂质体—硫代磷酸化修饰的c erbB 2反义寡脱氧核苷酸 (ASODN )作用后的卵巢上皮癌细胞株SKOV3 在裸鼠皮下的成瘤能力。方法 利用脂质体将经硫代磷酸化修饰的寡核苷酸导入SKOV3 细胞内, 然后将这种被转染的细胞接种于裸鼠皮下, 观察肿瘤体积的变化, 并计算抑瘤率。结果 经脂质体 c erbB 2ASODN作用后的卵巢上皮癌细胞在裸鼠皮下成瘤能力降低, 最大抑瘤率达 4 0 .0 %(P <0 .0 5 )。首次出现目检可见肿瘤的平均时间延长为 9.6天 (P <0 .0 5 )。结论 c erbB 2癌基因的反义调控能降低卵巢上皮癌细胞的成瘤能力, 抑制肿瘤生长, 在卵巢上皮癌的基因治疗中有一定价值。Abstract: Objective To explore the inhibitory effect of liposome-C-erbB-2 antisense phosphorothioate oligodeoxynucleotides (c-erbB-2 ASODN) on the human ovarian epithelial cancer transplanted subcutaneously in nude mice. Methods Human ovarian epithelial cancer cell lines SKOV 3 were treated with liposome -c-erbB-2 ASODN, and then transplanted subcutaneously in nude mice. The changes of tumor volume were observed and the tumor growth inhibitory rate was calculated. Results The tumorigenic ability of the t...
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