胃肠道间质瘤中PCNA和bcl-2蛋白的表达及临床意义
Expression of PCNA and bcl-2 in Gastrointestinal Stromal Tumors
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摘要: 目的探讨胃肠道间质瘤(gastrointestinalstromaltumor,GIST)中增殖细胞核抗原(PCNA)和bcl-2蛋白的表达与其发生及恶性转化的关系。方法采用免疫组化SP法及彩色病理图像分析系统检测68例GIST组织中PCNA和bcl-2蛋白的表达。结果68例GIST中,PCNA均呈阳性表达,其平均灰度值在良性组为144.95±3.37,潜在恶性组为139.54±4.91,恶性组为135.71±6.11,依次递减,即阳性表达水平逐渐增强,三者两两比较,差异均有显著性(P<0.05)。68例GIST中,bcl-2阳性表达者35例(51.47%),其平均灰度值在良性组为144.32±3.80,潜在恶性组为142.35±12.30,恶性组为140.35±12.06,三者两两比较,差异无显著性(P>0.05)。GIST中PCNA和bcl-2表达无明显相关性(P>0.05)。结论细胞的增殖与凋亡的失衡是GIST发生恶性转化的重要机制之一。PCNA检测有助于判断GIST的性质;bcl-2在GIST早期阶段就参与了肿瘤的发生发展,但不能作为判断良恶性的指标。Abstract: Objective To investigate the expression of PCNA and bcl-2 in gastrointestinal stomal tumors(GIST) and their correlations with the pathogenesis,development and differentiation degree of GIST. Methods The expression of PCNA and bcl-2 were detected in 68 cases of GIST by immunohistochemical technique.Futhermore,multi-function precise color-image measure system was used for quantitative analysis. Results All cases were positive for PCNA,the average gray in benign,potentially malignance and malignance were 144.95±...
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