Abstract: Objective To investigate the relationship between the expression of survivin，bcl-2，HSP90 protein and apoptosis in non-small cell lung cancer(NSCLC)． Methods Western blot to detect the expression of survivin，bcl-2，HSP90 protein in 80 cases of NSCLC and 20 cases of benign lung lesion. TUNEL assay was applied to detect cell apoptosis. Results Survivin was expressed in 57 of 80 (71.25%) cases of NSCLC and in 0 of 20 (0%) cases of benign lung lesion . There was negative correlation between the expression of survivin and apoptosis index. The positive rate of bcl-2 in lung carcinoma was 56.25% (45/80), much higher than that in benign lung lesion (10%) and the level of expression of bcl-2 and HSP90 in NSCLC was higher than that in benign lung lesion. There was significant correlation among the expression of survivin,HSP90 and bcl-2 in NSCLC． Conclusion survivin protein overexpression plays an important role in the occurrence and progression of NSCLC．There was significant correlation between the expression of survivin and the reducing of cell apoptosis.survivin may be identified as a new therapeutic target．The expression of survivin，bcl-2 and HSP90 was positive correlative, they cooperated in the occurrence and progress of NSCLC．