PCNA表达与肺癌细胞株凋亡的关系

Relationship between PCNA expression and apoptosis of human lung cancer cell line

摘要: 目的　探讨肺癌细胞株增殖细胞核抗原 (PCNA)的表达与肺癌细胞株凋亡的关系。方法　用三氧化二砷 (As2 O3 )诱导肺癌细胞株GLC 82凋亡。运用细胞培养、MTT、流式细胞技术 (FCM )检测及逆转录聚合酶链式反应 (RT PCR)检测PCNA表达在细胞凋亡过程中的变化及相互关系。结果　三氧化二砷可明显抑制GLC 82细胞增殖, 使细胞周期阻滞于G2 /M并随后凋亡, PCNA表达受到抑制。结论三氧化二砷诱导GLC 82凋亡过程中, PCNA表达受到明显抑制。对于耐药性肿瘤, PCNA活性的抑制可能代表一种新的化疗策略。

Abstract: Objective To investigate the relationship between proliferating cell nuclear antigen (PCNA) expression and apoptosis of human lung cancer cell line. Methods Arsenic trioxide (As_2O_3) was used to induce the direct apoptosis in human lung cancer GLC-82 cell line. The MTT method and flowcytometry (FCM) were used for detection. With RT-PCR, the changes and relations of PCNA in the process of GLC-82 apoptosis were studied. Results The data showed that As_2O_3 significatly. inhibited proliferation of GLC-82 ...