IFN-β基因诱导裸鼠胶质瘤Fas 表达上调及细胞凋亡的实验研究
Study of IFN-β Gene Inducing Nude Mice of Human SHG44 Glioma Up-regulating of Fas and Cell Apoptosis
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摘要: 目的 研究β干扰素基因对人脑胶质瘤的诱导凋亡作用,以及能否诱导胶质瘤细胞Fas表达上调,并探讨了β干扰素基因诱导细胞凋亡及Fas表达上调之间的联系. 方法 建立裸鼠SHG44胶质瘤模型,用脂质体包埋法将IFN-β基因的真核表达载体pSV2IFNβ注入裸鼠皮下SHG44脑胶质瘤,观察肿瘤生长情况并计算肿瘤体积,通过免疫组化、TUNEL染色,了解IFN-β基因诱导人脑胶质瘤细胞凋亡的作用以及胶质瘤细胞Fas表达情况. 结果 IFN-β在荷瘤裸鼠瘤体内获得表达,裸鼠皮下胶质瘤生长受到抑制,并诱导SHG44胶质瘤细胞凋亡,胶质瘤细胞Fas表达明显高于对照组. 结论 IFN-β基因能够抑制人脑胶质瘤生长,并诱导胶质瘤细胞凋亡;IFN-β基因诱导胶质瘤细胞凋亡可能是Fas表达上调的结果.Abstract: Objective To study the effect s of interferon-β( IFN-β) gene inducing human gliomas apoptosis and to observe whether interferon-βcan induce up-regulating of Fas expression. The relationship between cell apoptosis inducing by IFN-β gene and up-regulating of Fas expression was discussed. Methods To establish the SHG44 glioma model of nude mice. Eukaryonic expression vector (pSV2 IFNβ) for IFN-β packaged by liposome was injected into SHG44 glioma of nude mice ;and observe the glioma growth and calculate tumor size. The effects of IFN2β gene inducing human gliomas apoptosis and Fas expression were realized by immunohistochemistry and tunel. Results The expression of IFN-β gene in the glioma nude mice were determined. The glioma growth of nude mice was inhibited. IFN-βcan induce glioma cell SHG44 apoptosis. Fas expression of glioma cell is significantly higher than that of the cont rol group. Conclusion IFN-βgene can inhibit the growth of human gliomas and induce the apoptosis of human glioma cells. The apoptosis inducing by IFN-β gene may depend on the up-regulating of Fas expression.
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