全反式维甲酸对结肠癌细胞XIAP相关因子1表达的影响
Effects of All-traps Retinoic Acid on Expression of XAF1 in Colon Cancer Cell Line
-
摘要: 目的了解全反式维甲酸对结肠癌lovo细胞XIAP相关因子1(XAF1)表达及细胞增殖的影响。方法以不同浓度的ATRA刺激lovo细胞,RT-PCR检测XAF1的mR-NA水平变化,Westernblot分析XAF1蛋白水平的表达;MTT法检测ATRA对lovo细胞生长抑制率。构建含有XAF1启动子序列荧火虫荧光素酶报告质粒,分析ATRA作用对XAF1启动子活性的影响。结果经ATRA作用,lovo细胞mRNA和蛋白的表达水平上调,细胞生长受到抑制。上述效应均具有药物浓度依赖性。报告基因分析结果显示,ATRA使含有XAF1启动子序列荧火虫荧光素酶报告质粒的虫荧光素酶的活性增加。结论ATRA通过促进启动子转录活性上调XAF1的mRNA和蛋白表达水平,并抑制结肠癌细胞的生长。Abstract: Objective To investigate the effect of all-trans rctinoic acid(ARTA) on XIAP-associated factor 1(XAF1) expression and the growth of human colorectal carcinoma. Methods Lovo cell were treated with various concentrations of ATRA.The mRNA and protein expression of XAF1 were det-ected by Western blot and RT-PCR methods.The cell growth was measured by MTT assay.Transcription activity of XAF1 promoter is examined by luciferase reporter assay. Results The mRNA and protein expression of XAF1 were up regulated by ATRA stimulation in a dose2dependent manner, MTT showed that ATRA could suppress the proliferation of colorectal carcinoma cells. ATRA stimulation also resulted in increase in luciferase activity in cells t ransfected with the pla-smid containing XAF1 promoter region sequences. Conclusion ARTA could significantly inhibit the growth of human color2ectal carcinoma cells, and this anti2tumor effect might be related to the up2regulation of XAF1 mRNA and protein by increasing the t ranscription activity of XAF1 promoter.
下载:
