SHP-1与SOCS3基因在成人急性白血病中 的表达及其与预后的关系

罗建民; 杨琳; 李燕; 刘小军; 温树鹏; 王福旭; 张敬宇; 张学军; 杜行严; 姚丽; 董作仁

doi:10.3971/j.issn.1000-8578.2009.05.007

SHP-1与SOCS3基因在成人急性白血病中 的表达及其与预后的关系

Clinical Value of the Expression of SHP-1 and SOCS3 Genes in Leukemia Cells

摘要

摘要: 目的了解SHP-1基因和SOCS3基因在白血病细胞中的表达及其对预后的影响，为进一步阐明SHP-1和SOCS3基因在白血病发病中的作用机制打下基础。方法用实时荧光定量PCR（FQ-PCR）和RT-PCR方法分别对AL患者和13例正常对照的骨髓进行SHP-1、SOCS3 mRNA水平监测。结果 ①正常人SHP-1 mRNA均呈阳性表达，其表达水平为3.413±2.018，ALL患者SHP-1均为阴性表达；初治、复发AL组SHP-1表达水平明显低于正常对照组(P＜0.01)，缓解后SHP-1 mRNA水平上升但仍低于正常对照(P＜0.01)。② 初治和复发AL患者SOCS3 mRNA表达水平均明显高于缓解组和正常对照组(P＜0.05)；缓解患者SOCS3 mRNA与正常对照相比无统计学意义(P＞0.05)。③ SHP-1表达阳性组缓解率（76.4%）明显高于表达阴性组（47.3％）(P＜0.01)。结论 SHP-1和SOCS3均参与白血病发病，但发病机制不同。SHP-1与白血病的疗效预后有关。

Abstract: Objective To investigate the role of SHP-1 and SOCS3 in leukemia and the effects on clinical prognosis. To furtherly clarify the effects of SHP-1 and SOCS3 gene on cell proliferation signal pathway. Methods We used FQ-PCR and RT-PCR to detect the mRNA level of SHP-1 and SOCS3 in AL patients and normal control respectively. Results ① The expression of SHP-1 mRNA in NC was positive（3.413±2.018） and it was significant lower in de novo AL than NC group (P＜0.01). CR was achieved and decreased again after the disease replased. The expression of SHP-1 in AML was significantly different with ALL(P＜0.001). ② The mRNA level of SOCS3 in de novo and relapsed AL was obviously higher than that of normal control(P＜0.05)，and there was no significant defference between CR group and NC group(P＞0.05). ③ The complete remission in SHP-1 positive patients（76.4%） was dramatically higher than those with negative SHP-1 expression(P＜0.01). Conclusion The SHP-1 and SOCS3 both effect on the leukemia, but the mechanism is different. SHP-1 is related with the prognosis of leukemia.

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