CXCR4/SDF-1α对宫颈癌HeLa细胞定向迁移及增殖的影响
Effect of CXCR4/SDF-1α in Derected Migration and Proliferation of HeLa Cells
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摘要: 目的了解CXCR4在HeLa细胞的表达情况,并借助细胞培养评价CXCR4/SDF-1α对HeLa细胞定向迁移及增殖的影响。方法CXCR4 mAb免疫染色HeLa细胞。用Transwell侵袭转移模型评价HeLa细胞的迁移情况,其中,上室中加入预先用(或不用)CXCR4单抗预孵育的HeLa细胞,下室中加入含0~100ng/mlSDF-1α的培养基。为评价CXCR4、SDF-1α对HeLa细胞增殖的影响,将HeLa细胞接种于有(无)SDF-1α和(或)CXCR4的低血清环境72h。结果CXCR4在所有HeLa细胞上均有表达。HeLa细胞能顺SDF-1α浓度差定向迁移,且这一作用可被CXCR4 mAb拮抗。SDF-1α能促进He-La细胞在低血清环境中增殖。结论CXCR4/SDF-1α参与了HeLa细胞定向迁移的过程并影响其增殖。Abstract: Objective To investigate the expression of CXCR4 in HeLa cells,and evaluate the effect of CXCR4/ SDF-1α in directed migration and proliferation of HeLa cells. Methods HeLa cells were immunohistochemically stained with mouse anti-CXCR4 monoclonal antibody (mAb).In vitro invasion of HeLa cells was evaluated using Transwell Permeable Supports,in which HeLa cells with/without CXCR4 mAb pre-incubation were seeded in the upper chambers,medium containing 0～100ng/ml of SDF-1α was added to the lower compartments.For evaluating the effect of CXCR4/ SDF-1α on proliferation of cervical cancer cells,HeLa cells were cultured for 72 hours exposed to SDF-1α with and without CXCR4 mAb. Results CXCR4 was expressed on all HeLa cells.SDF-1α induced the directed migration of HeLa cells with a concentration-depended model,which was inhibited by using CXCR4 mAb.SDF-1α also stimulated the proliferation of HeLa cells mediated by CXCR4. Conclusion CXCR4/ SDF-1α participate in the directed migration and proliferation of HeLa cells.
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