p14<sup>ARF</sup>在宫颈癌中的表达及其与p53 表达相关性的研究

p14^ARF在宫颈癌中的表达及其与p53 表达相关性的研究

摘要: 目的　探讨宫颈癌组织p14^ARF蛋白的表达及其与p53 表达的相关性。方法　应用免疫组化方法检测p14^ARF 、p53 基因在41 例宫颈癌及20 例正常宫颈组织中的表达。结果　p14^ARF在正常宫颈组织中不表达,41 例宫颈癌中35 例表达阳性,占85. 4 %。病理分级为G1 、G2 级的宫颈癌的p14^ARF阳性表达率为68. 4 %, G3 级为100 %, 两者比较,有显著性差异( P < 0. 05) 。宫颈癌组织中p53 蛋白表达阳、阴性者中p14ARF蛋白表达阳性率分别为75. 0 %(12/ 16) 和92. 0 %(23/ 25), 两者比较,无显著性差异,p14^ARFF与p53 表达不相关。结论　p14^ARF在宫颈癌中高表达有一定的诊断和估测预后价值,可能是宫颈癌新的肿瘤标志。

Abstract: Objective 　To study the expression of p14^ARF protein in cervical carcinoma and it s relationship with the expression of p53 gene. Methods 　The expression of p14^ARFand p53 protein was detected by immunohistochemist ry in 41 cervical carcinoma tissues and 20 normal cervical tissues. Results p14^ARFwas expressed in 35 of 41 (85. 4 %) cases of cervical carcinomas. In cont rast, normal cervical epithelium did not express p14^ARF . Overexpression of p14^ARF was related to the tumor grade. The expression of p14^ARF had no association with p53. Conclusion 　The overexpression of p14^ARF has value in diagnostic and prognosis in cervical neoplasm. These proteins maybe use as new markers of cervical neoplasm.