乳腺癌组织中Livin mRNA与Smac蛋白的表达
Expression of Livin mRNA and Smac in Breast Cancer
-
摘要: 目的 探讨凋亡抑制因子Livin mRNA和凋亡促进因子Smac蛋白在人乳腺癌的表达及相关性，分析其与乳腺癌转移复发的关系。方法 收集50例乳腺癌和相应的癌旁组织，应用逆转录聚合酶链反应技术和免疫组织化学法检测组织中Livin mRNA与Smac的表达水平。结果 50例乳腺癌组织中Livin mRNA表达的阳性率显著高于相应的癌旁组织（P＝0.000），癌组织中Smac表达的率显著低于癌旁组织（P＝0.008）。Livin mRNA的表达与TNM分期、腋窝淋巴结转移、术后复发及C-erbB-2表达密切相关（P<0.05）。Smac的表达与TNM分期、组织学分级、腋窝淋巴结转移、术后复发及C-erbB-2密切相关（P<0.05）。Livin mRNA和Smac的表达有显著负相关性（γ=-0.357，P=0.011）。结论 Livin mRNA的高表达及Smac的失活可能在乳腺癌的发生及进展中起着重要作用。Abstract: Objective To investigate the expression of Livin mRNA and Smac protein in breast cancer and their correlations with metastasis and recurrence. Methods The expression of Livin mRNA was investigated by reverse transcriptase polymerase chain reaction(RT-PCR) in 50 breast carcinomas and paired adjacent breast tissues. The expression of Smac protein in the same cases was detected by immunohistochemestry (IHC). ResultsLivin mRNA was over expressed in breast cancers compared with paired adjacent breast tissues and Smac protein was significantly lower that compared with that expressed in paired adjacent breast tissues. The expressions of Livin mRNA was significantly correlated with the TNM stage, axillary lymph node metastasis, the recurrence of tumor and the express of C-erbB-2. The protein expression of Smac was significantly correlated with histological grade, the TNM stage, axillary lymph node metastasis, the recurrence of tumor and the express of C-erbB-2. There was a significantly negative relationship of Livin with Smac. Conclusions The inactivation of Smac protein and overexpression of Livin mRNA might enhance the tumor cell proliferation and malignance,and might play an important role in the occurrence and development of human breast cancer.
下载:
