高表达p73 基因对肺腺癌细胞VEGF 、bFGF 表达抑制作用的研究

Overexpression p73 Gene Inhibit VEGF, bFGF Expression in Lung Adenocarcinoma Cell

摘要: 目的观察高表达的p73基因对肺腺癌细胞中VEGF、bFGF mRNA和蛋白表达水平的影响。方法将p73基因以脂质体法转染A549细胞、H1299细胞，G418筛选阳性细胞克隆．采用RT-PCR半定量法检测转染前后VEGF、bFGF mRNA的表达水平，Western-blot半定量法检测蛋白表达水平。结果转染p73基因后，A549细胞、H1299细胞中VEGF、bFGF mRNA和蛋白表达水平均下降，较未转染p73基因的细胞有显著性差异(P<0．05)。结论高表达的p73基因能够降低肺腺癌细胞中VEGF、bFGF mRNA和蛋白表达水平，提示p73基因可能抑制人类肺腺癌的血管生成。

Abstract: Objective 　To study the effect of overexpression p73 gene on VEGF, bFGF expression in lung adenocarcinoma cell. Methods 　p73 gene was t ransferred into A549 cell and H1299 cell by liposome and the positive cell clones were chosen by G418. The VEGF and bFGF mRNA expression were detected by RT-PCR, protein expression were detected by western-blot . Results 　The VEGF, bFGF mRNA and protein expression in A549 cell and H1299 cell were decreased af ter t ransferred p73 gene ( P < 0. 05) . Conclusion 　Overexpression p73 gene can inhibit the VEGF and bFGF expression in lung adenocarcinoma, so it can inhibit the angiogenesis in lung adenocarcinoma.