Abstract: Objective To study immunohistochemical expression of Glut-1 and COX-2 in endometrial adenocarcinoma and precancerous lesion. Methods Immunohistochemical staining S-P method was used to detect the expression of Glut-1 and COX-2 in 20 cases of normal proliferative endometrium,23 cases of simple endometrial hyperplasia,21 cases of complex endometrial hyperplasia,43 cases of atypical endometrial hyperplasia,25 cases of endometrial adenocarcinoma. Results The positive expression rates of Glut-1 and COX-2 were 0% and 10. 0 % in normal proliferative endomet rium, 0 % and 9. 1 % in simple endomet rial hyperplasia, 4. 8 % and 14. 3 % in complex endomet rial hyperplasia, 20. 0 % and 25. 0 % in low2grade atypical endomet rial hyperplasia, 52. 2 % and 52. 4 % in high2grade atypical endomet rial hyperplasia, 88. 0 % and 91. 7 % in endomet rial adenocarcinoma. There were all significant difference between benign, hyperplastic and malignant endomet rial epithelia, the difference between endomet rial adenocarcinoma and high2grade atypical endomet rial hyperplasia were also statistically significant . Positive correlation was also found between COX22 and Glut21 expression ( P = 0. 000) . Conclusion 　Abnormal expression of Glut21 and COX22 may cont ribute to the pathogenesis and development of the endomet rical carcinoma, the combined use of Glut21 and COX22 is valuable in distinguishing endomet rial adenocarcinoma f rom endomet rial hyperplastic lesions.