Abstract: Objective 　To explore the expression and clinical significance of P-gp 、GST-Лand p53 and CD44v6 gene protein in human lung cancer. Methods 　The expression of P-gp 、GST-Лp53 and CD44v6 protein were detected in 60 cases of lung cancer tissue with SP immunohistochemical method and analyzed. Results 　The result showed that : ① the positive expression of P-gp and GST-Лseparately were 69. 8 % and 73. 6 % in NSCLC, which were much higher than the positive expression in SCLC(14. 3 %) ( P <0.05), compared with adenocarcinoma and squamous carcinoma 、SCLC, the positive rate of P-gp and GST-Л in adenocarcinoma were higher ( P < 0. 05) . ②The positive rate of CD44v6 gene production is 65. 0 %, which has a significant positive correlation with lymph node metastasis ( P < 0. 05) . ③The coexpression rate of P-gp and GST-Л is 41. 7 %, P-gp significantly positively correlated with GST-Л. The coexpression rate of P-gp and p53 is 48. 3 %, P-gp had obviously positive correlation with p53. Conclusion 　The overexpression of P-gp 、GST-Лand p53 in human lung cancer made it important to influence tumor resistance. The over-expression of CD44v6 may be the good indicator for predicting metastasis and prognosis of lung cancer.