白龙灵沙汤提取物对A549人肺癌细胞凋亡的影响

Apoptosis of Human Lung Cancer Line A549 Induced by Bailonglingshatang Extract

摘要: 目的探讨白龙灵沙汤提取物对A549人肺癌细胞凋亡的影响。方法提取白龙灵沙汤中的有效成分，使之与A549人肺癌细胞作用48、72h后，免疫细胞化学法检测 bcl-2、bax表达。结果白龙灵沙汤提取物与A549人肺癌细胞作用 48、72h后，下调A549人肺癌细胞 bcl-2，上调 bax的表达。结论白龙灵沙汤提取物可下调 bcl-2和上调bax的表达。

Abstract: Objective To observe the effect of Bailonglingshatang(BLLST) extract on apoptosis of human lung cancer cell line A549. Methods The human lung cancer cell line A549 was in vitro co-cultured with BLLST extract for 48 and 72h,respectively.Immunocytochemical method was used to detect the expression of bcl-2 and bax. Results The expression of bcl-2 was down-regulated and the expression of bax was up-regulated in A549 cells after treated by the BLLST extract. Conclusion BLLST extract can restrain lung cancer cell multiplication,down-regulate the expression bcl-2 and up-regulate the expression bax.