Abstract: Objective To investigate the expression of Twist and IGF-1 in colorectal carcinomas and the relation with clinical and pathological factors,and to analyze the interaction of Twist and IGF-1 on nosogenesis of colorectal carcinomas and the prognosis of patients. Methods Immunohistochemical SP methods was adopted to examine the expression of Twist and Immunohistochemical SABC methods was adopted to examine the expression of IGF-1 in colorectal carcinomas（48 cases） and paracancerous normal tissues(24 cases). Results The positive rates of Twist and IGF-1 in colorectal carcinomas(47.92% and 64.58%) were significantly higher than those in paracancerous normal tissues (12.50% and 16.67%). The overexpression of Twist and IGF-1 were significantly related with the tumor differentiation,the depth of invasion,lymph node metastasis and the Dukes stage. The overexpression of Twist was aslo significantly related with the 5 year survival state. The expression of Twist in colorectal carcinomas was obviously related with IGF-1. Conclusion The expression of Twist and IGF-1 may play a cooperative role in the infiltration and metastasis of colorectal carcinomas.The positive expression of Twist indicates poor prognosis and provide a valuable reference for the decision of prognosis.