膀胱肿瘤患者外周血淋巴细胞p16和cyclin D_1的表达及其临床意义

Expression of p16 and cyclin D_1 protein in peripheral blood of patients with bladder cancer

摘要: 目的　研究膀胱肿瘤患者外周血中淋巴细胞p16和cyclinD1的表达及其临床意义。方法　应用FCM检测 4 7例膀胱癌及 2 0例对照组的外周血淋巴细胞中 p16和cyclinD1的表达。结果　膀胱癌患者p16和cyclinD1基因蛋白的平均表达率分别为 (2 .4 8± 0 .91) %、(12 .4 8± 9.2 7) %与正常人群的表达率 (7.4 9± 5 .2 2 ) %、(0 .6 6± 0 .5 ) %比较有显著差异 (P <0 .0 1) ;治愈 37例中 p16和cyclinD1的表达率正常, 而复发者 p16和cyclinD1无一例在正常值以内。结论　外周血淋巴细胞 p16和cyclinD1的异常表达与荷瘤状况有密切关系, 有望为分子诊断与筛选提供依据, 并可作为膀胱癌复发的监测指标。

Abstract: Objective To investigate the expression of p16 and cyclin D_1 protein in peripheral blood of bladder cancer. Methods p16 and cyclin D_1 protein were determined with flow cytometry in 47 cases bladder cancer and 20 cases normal control. Results The levels of p16 were ( 2.48 ± 0.91 )% in cancer group and ( 7.49 ± 5.22 )% in control group. While the levels of cyclin D_1 were ( 12.48 ± 9.27 )% and ( 0.66 ± 0.5 )%. Mean value of p16 in the cancers were lower than that in the controls ( P < 0.01 ),...