AML-M_5细胞诱导TCR Vβ T细胞克隆性增殖研究
Clonal expansion of TCR Vβ subfamily T cells induced by AML-M_5 cells
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摘要: 目的 了解急性髓细胞白血病 (AML)M5亚型细胞诱导T细胞的TCRVβ亚家族限制性利用和克隆性增殖情况。方法 采用混合淋巴细胞 /肿瘤细胞培养 (MLTC)方法, 用AML M5细胞体外诱导供者外周血和脐血的T细胞增殖, 用RT PCR扩增经MLTC前后获得细胞的 2 4个TCRVβ亚家族基因的互补决定区 (CDR3), 了解病人各Vβ亚家族的利用情况。并经基因扫描分析确定M5细胞诱导的T细胞克隆性特点。结果 脐血和供者外周血分别表达 13和 5个TCRVβ亚家族T细胞, 经MLTC培养初期, 则表达全部 2 4个Vβ亚家族, 随着诱导时间的增加, 诱导T细胞所表达的Vβ亚家族逐渐减少。基因扫描显示AML M5细胞诱导后的T细胞出现寡克隆和双克隆增殖改变, 主要为Vβ2 1的克隆性增殖T细胞。结论 AML M5细胞可诱导TCRVβT细胞克隆性增殖, 这可能是对AML M5细胞相关抗原的特异细胞免疫应答的结果。Abstract: Objective To investigate the restricted usage and clonal expansion of TCR Vβ subfamily T cells induced by AML-M_5 cells. Methods The mononuclear cells(MNC) from donor peripheral blood or cord blood were amplified by mixed lymphocyte and tumor culture(MLTC), induced by AML-M_5 cells from 2 patients respectively. The complementarity determining region 3(CDR3) of TCR β with variable region genes was amplified in T cells from the donor or cord blood before and after MLTC using RT-PCR, to detect the usage feat...
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