视黄酸依赖Fas/RARα融合基因转染MCF-7细胞启动的凋亡效应研究

Apoptosis of MCF-7 cells induced by fusion gene of Fas/RARα

摘要: 目的　检测视黄酸依赖Fas/RARα表达载体转染MCF 7细胞启动的凋亡效应。方法　构建视黄酸依赖Fas/RARα表达载体, 经脂质体转染MCF 7细胞, 经MTT、流式细胞术、DNA梯形带和TUNEL等方法观测视黄酸处理后细胞凋亡效应的发生。结果　以一定剂量视黄酸处理MCF 7后, 细胞增殖受抑, 呈凋亡性变。结论　视黄酸及其依赖的融合基因表达可协同诱导MCF 7细胞凋亡效应

Abstract: Objective To test the apoptosis of MCF-7 cells after transfection of the vector of Fas/RARα fusion gene induced by ATRA. Methods After transfection of fusion gene vector pRARE-Fas/RAR alpha and treatment of RA in tumor cells, the cellular proliferation capacity was observed with MTT assay, the cell cycle distribution and the apoptotic rate were measured with flow cytometry, DNA ladder and TUNEL. Results The proliferation capacity was decreased, the apoptotic rate was increased in MCF-7 cells treated by R...