三种反义c-myc RNA 稳定表达对HL-60细胞生长、增殖的影响靶细胞生长、增殖的影响。
The Effects of Three Antisense c-myc RNA Tranquilization Expression on the Gronth and proliferation of HL-60
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摘要: 目的 比较不同的反义 c- myc基因导入对 HL- 60生长、增殖的影响。方法 将分别载有 c- myc等 1、2和 3外显子反义片段的三种重组逆转录病毒表达载体 a M1、a M2和 a M3导入体外培养的 HL- 60, 观察对靶细胞生长、增殖的影响。结果 ( 1 )三种载体抑制了 c- Myc的表达, 抑制作用 a M2 >a M1 >a M3;( 2 ) a M2使 HL- 60 Go/G1细胞增多, S期减少, DNA和总蛋白合成减少 ;a M1影响轻微 ;a M3作用相反 ;( 3) a M2使 HL- 60 PCNA表达减少 43.8%, 而 a M1和 a M3增强表达 ;( 4 ) a M2明显抑制细胞的生长、增殖活性和软琼脂集落形成能力 ;a M1作用相反 ;a M3抑制生长、增殖活性, 但促进其集落形成能力。结论 a M2持续表达抑制了 HL- 60的生长、增殖.Abstract: Objective To compare the effects of different antisense c-myc molecule on the growth and proliferation of HL-60. Methods Three antisense retrovirus expression vectors for human c-myc exon 1,2 and 3, respectively named aM1, aM2 and aM3, were tranfered into cultured HL-60 cells. Results (1)Stable expression of the three vectors reduced HL-60 Myc expression(aM2>aM1>aM3);(2)aM2 increased the ratio of G0/G1, decreased the proportions of S phase, DNA and total protein synthesis. However, little change ...
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