用免疫组化和原位杂交方法检测和分析人骨肉瘤组织中Ⅰ、Ⅱ和Ⅲ 型胶原蛋白和mRNA 表达

Study of Prote in and mRNA of Type Ⅰ、Ⅱ and Ⅲ Collagen in Human Osteosarcoma by Immunohistochemistry and in Situ Hybridization

摘要: 目的　观察骨肉瘤组织、和型胶原蛋白和 m RNA的表达及其与骨肉瘤分型和分化的关系。方法　免疫组化 LSAB法检测胶原蛋白, 原位杂交检测胶原 m RNA的表达, 用图像分析系统进行分析和半定量测定。结果　骨肉瘤组织不仅表达型胶原蛋白 (阳性率 87%, 灰度值1 71 .99± 1 4.74), 并出现了型和型胶原蛋白 (阳性率分别为 30 .4%和 43.4%, 灰度值分别为1 5 3.0 7± 1 8.82和 1 68.2 9± 1 8.36) ;软骨母细胞型和混合型骨肉瘤型胶原的表达高于其它型, 差异有显著性 ( P<0 .0 1 ) ;高中低分化的三组骨肉瘤间, 型胶原表达差异有显著性 ( P<0 .0 1 ), 分化越低表达越少。、型胶原 m RNA表达与蛋白表达所得结论基本一致。结论　型胶原可作为软骨母细胞型和混合型骨肉瘤的分型参考指标, 型胶原可作为骨肉瘤分化及恶性度判断的参考指标.

Abstract: Objective To investigate expression of type Ⅰ?Ⅱ and Ⅲ collagen in human osteosarcoma and their relationship with classification and differentiation of osteosarcoma. Methods The protein and mRNA of type Ⅰ?Ⅱ and Ⅲ collagen in 46 cases of osteosarcoma were detected using immunohistochemistry (LSAB) and in situ hybridization;EMAL 200 image software was used to analyse staining result. Results Besides type Ⅰ collagen (positive rate 87%,grey value 171.99±14.74),there were also expression of type Ⅱ and Ⅲ collagen i...