COX-2 基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性研

吴　涛; 袁先厚; 文志华; 马　超; 吴志敏

doi:10.3971/j.issn.1000-8578.1267

COX-2 基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性研

Expression and Relationship of Cyclooxygenase-2 and Matrix Metalloproteinase in human Astrocytoma

摘要

摘要: 目的探讨COX-2基因与基质金属蛋白酶在星形细胞瘤中的表达及二者相关性。方法用原位杂交的方法检测星形细胞瘤组织中COX-2 mRNA的表达；用Ultrasensitive S-P法(链霉素抗生物素蛋白-过氧化物免疫组化染色)检测同等标本中基质金属蛋白酶-2和基质金属蛋白酶-9蛋白的表达；并将COX-2 mRNA与基质金属蛋白酶的表达作相关性分析。结果 COX-2 mRNA及MMP2、MMP9在星形细胞瘤中高度表达，表达阳性率分别为62．69％、56．70％和58．20％；COX-2 mRNA与MMP2和MMP9均有正相线性关系，Pearson积距相关系数分别为0．260、0．347，P<0．05。结论 COX-2基因和基质金属蛋白酶在星形细胞瘤组织中的表达密切相关，说明它在星形细胞瘤向周围组织侵袭中起重要作用。

Abstract: Objective 　Investigate expression and relationship of Cyclooxygenase-2 and matrix metalloproteinase (MMP) in human ast rocytoma. Methods 　In this study, COX-2 mRNA was investigated in 67 astrocytomas and 8 normal brain specimens by using in situ hibrid-ization study. Metalloproteinase-2 (MMP2) and metalloproteinase-9 (MMP9) expression was investigated in 67 ast rocytomas and 8 normal brain specimens by immunohistochemist ry. Results 　Expression of COX-2 mRNA, MMP2 and MMP9 was observed in ast rocytoma and not in normal brain specimens. The expression rate of COX-2 mRNA, MMP2 and MMP9 were 62. 69 %、56. 70 % and 58. 20 %. Conclusion 　Ast rocytoma invasion st rongly connects with COX-2 gene. The study suggest s that COX-2 gene may perform an important role in human astrocytoma invasion.

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