Abstract: Abstract:Objective To investigate the relationships among Oct4 and Wnt/β-catenin as well as TGF-β signal ways in hepatocellular carcinoma(HCC) cell line HepG2. Methods RT-PCR was used to detect the expression of Oct4 and the genes in both Wnt/β-catenin and TGF-β signal ways in HCC cell lines and HCC specimens. RNAi was used to mediated knock-down the expression of Oct4 and TCF3 in HepG2, and the change of Wnt/β-catenin and TGF-β related genes were also detected by RT-PCR. Results Oct4 and the genes in both Wnt/β-catenin and TGF-β signal ways including β-catenin, Wnt10b, TCF3 and ELF, Smad3, Smad4 were expressed in HCC cell lines as well as in the specimens from HCC patients. After treating the Oct4 in HepG2 with siRNA, the expression of TGF-β family genes ELF, Smad3, Smad4 and Wnt/β-catenin family genes wnt10b,β-catenin reduced,while TCF3 increased. In reverse, knockingdown TCF3 led to an increase in the expression of Oct4 and TGF-β family genes. Conclusion The result of silencing Oct4 and TCF3 in HepG2 by siRNA suggested that there is a certain correlation among Oct4，Wnt/β-catenin and TGF-β family genes in HCC cell line HepG2, which reveals the role of interactions between Oct4 in HCC is more worthy to be researched.