多发性骨髓瘤p15、p16基因启动子区甲基化的研究

Hypermethylation of CPG island of p15 and p16 genes in multiple myeloma

摘要: 目的探讨ｐ１５、ｐ１６基因启动子区ＣＰＧ岛甲基化在多发性骨髓瘤发病中的作用。方法利用甲基化特异性ＰＣＲ技术，研究骨髓瘤细胞株（Ｕ２６６、ＬＰ１、ＫＭ３）及多发性骨髓瘤患者ｐ１５、ｐ１６基因甲基化状态。结果Ｕ２６６、ＬＰ１细胞ｐ１５、ｐ１６基因处于完全甲基化状态；ＫＭ３细胞则为完全未甲基化模式；ＭＭ患者的ｐ１５、ｐ１６基因启动子区甲基化比例分别为５２．３８％及５７．１４％。ｐ１５、ｐ１６基因甲基化与ＭＭ分期无关（Ｐ＞０．０５）。结论ｐ１５、ｐ１６基因甲基化在多发性骨髓瘤中较为常见，这可能为多发性骨髓瘤的治疗提供借鉴。

Abstract: Objective To illustrate the role of hypermethylation of p15 and p16 genes in the pathogenesis of multiple myeloma. Methods By using methylation-specific PCR (MSP) technique,the methylation status of p15 and p16 genes in MM were analysed. Results p15 and p16 gene are completely methylated . However both p15 and p16 genes are unmethylated in KM3 cells. Hypermethylation of CPG island was observed in 52. 38% for p15 and 57. 14% for p16 in our group of MM patients. There were ...