Abstract:
Objective To explore the gene regulation mechanism of SCLC, so as to provide a basis for the early diagnosis and targeted treatment of SCLC with potential biomarkers.
Methods SCLC mRNA data were obtained from the public Gene chip database and DEGs were screened out. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted on DEGs. A protein interaction network was constructed. Core genes were screened out and Kaplan-Meier online tool was used for survival analysis.
Results A total of 248 differentially-expressed genes, including 172 up-regulated genes and 76 down-regulated genes, were screened out from 17 cases of small cell lung cancer tissue samples and 19 cases of normal tissue control samples (P < 0.05). The GO and KEGG enrichment analysis results showed that the functions of DEGs mainly involved in cell cycle, DNA replication, mismatch repair, p53 signaling pathway, etc. Through the protein interaction analysis network, the core genes with the highest degree of 6 nodes were selected: TOP2A, PCNA, RFC4, FEN1, CCNA2 and MCM2, which were related to the prognosis of patients.
Conclusion The molecular functions and signaling pathways involved in DEGs may be the molecular mechanism of the occurrence of SCLC, and core genes may be potential targets for the treatment of SCLC.
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