BCYRN1对乳腺癌细胞MCF7和小鼠移植瘤增殖和转移的影响

皮亚平; 刘黎明

doi:10.3971/j.issn.1000-8578.2018.17.0691

BCYRN1对乳腺癌细胞MCF7和小鼠移植瘤增殖和转移的影响

Influence of BCYRN1 on Proliferation and Migration of Breast Cancer Cell Line MCF7 and Tumor-bearing Mice

摘要

摘要:
目的研究RNA干扰lncRNA BCYRN1对乳腺癌细胞MCF7和小鼠移植瘤的增殖和转移的影响。
方法 BCYRN1 siRNA和siRNA scramble（对照组）分别转染乳腺癌细胞MCF7。CCK-8检测细胞增殖；蛋白印迹法检测细胞中蛋白的表达；划痕实验分析细胞运动能力；免疫组织化学法检测肿瘤组织中蛋白的表达。
结果与对照组相比，细胞增殖倍数明显降低（P=0.02）；且细胞增殖核抗原-67（antigen identified by monoclonal antibody, Ki-67）和增殖细胞核抗原（proliferating cell nuclear antigen, PCNA）表达显著减弱（P=0.03）。BCYRN1 siRNA处理后，乳腺癌MCF7细胞运动能力减弱，血管内皮细胞生长因子（vascular endothelial growth factor, VEGF）和基质金属蛋白酶9（matrix metalloprotein 9, MMP-9）表达受到抑制（P=0.04）。体内实验表明，BCYRN1 siRNA组小鼠肿瘤的体积明显小于对照组（P=0.03），且BCYRN1 siRNA可以抑制MCF7小鼠移植瘤肿瘤组织Ki-67和MMP-9的表达。
结论 BCYRN1 siRNA可以抑制乳腺癌细胞MCF7和小鼠的增殖和转移。

Abstract:
Objective To investigate the effect of BCYRN1 on proliferation and migration of breast cancer cell line MCF7 and transplatation tumor in mice.
Methods The breast cancer cell line MCF7 was transfected with BCYRN1 siRNA or siRNA scramble. Cell proliferation folds were tested by CCK-8. Protein expression was measured by Western blot. Wound healing assay was used to detect cell migration. The expression of proteins in breast cancer tissues was checked by immunohistochemistry staining.
Results Compared with control group, the cell proliferation folds in BCYRN1 siRNA group was attenuated (P=0.02). Moreover, the expressions of antigen identified by monoclonal antibody (Ki-67) and proliferating cell nuclear antigen (PCNA) were decreased (P=0.03). BCYRN1 siRNA treatment reduced the migration of breast cancer cells and repressed the expression of vascular endothelial growth factor (VEGF) and matrix metalloprotein 9 (MMP-9) (P=0.04). In vivo, the tumor volume of BCYRN1 siRNA group was smaller than that of control group (P=0.03); furthermore, the expression of Ki-67 and MMP-9 were decreased by BCYRN1 siRNA.
Conclusion BCYRN1 siRNA could suppress the proliferation and migration of breast cancer cell line MCF7 and transplantation tumor in mice.

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